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URL: http://www.addgene.org/35964
Proper Citation: RRID:Addgene_35964
Insert Name: Tie2 promoter
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9096345
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescriptII SK (+); Vector Types:; Bacterial Resistance:Ampicillin
Comments: Positive control DNA where LacZ is expressed under the control of Tie2 promoter and minimum enhancer fragment. This plasmid is unstable in bacteria. The depositing laboratory recommends performing minipreps, as larger scale preps tend to result in recombination. To verify the plasmid, perform separate digests with KpnI and HindIII. KpnI should produce two bands at approximately 7kb and 3kb. HindIII should produce two bands at approximately 8kb and 2kb. See Addgene's diagnostic digest as an example.
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Source: Addgene
