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URL: http://www.addgene.org/36158
Proper Citation: RRID:Addgene_36158
Insert Name: Sec23A
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:21045114
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLVX-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The cDNA sequence encoding mRuby (Kredel et al., 2009) underwent codon usage adaptation before gene synthesis (MWG Eurofins) and cloning into pBluescript II SK(+). mRuby was excised with SalI-EcoRI for insertion into pLVX-Puro (Clontech) to generate pLVX-Puro–mRuby. A cDNA encoding human Sec23A was amplified by PCR, gel purified and subcloned via pGEM-T vector (excised with ApaI-XbaI) and cloned into pLVX-Puro–mRuby.
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Source: Addgene
