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URL: http://www.addgene.org/36316
Proper Citation: RRID:Addgene_36316
Insert Name: ZifCY2 genetically encoded Zn(II) sensor
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19363034
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: First generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from mammalian Zif268. ZifCY2 is a variant of ZifCY1. The two cysteines (amino acids 237 and 242) in the Zn(II)-binding domain of ZifCY1 are mutated to histidines in ZifCY2.
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Source: Addgene