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URL: http://www.addgene.org/36317
Proper Citation: RRID:Addgene_36317
Insert Name: mito-ZifCY1 genetically encoded Zn(II) sensor
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19363034
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: First generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from mammalian Zif268. mito-ZifCY1 contains 4 tandem repeats of the first 29 aa of human cox subunit 8a (MSVLTPLLLRGLTGSARRLPVPRAKIHSL). HindIII restriction sites are at the 5' and 3' ends of the mitochondrial targeting sequence.
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Source: Addgene
