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URL: http://www.addgene.org/36405
Proper Citation: RRID:Addgene_36405
Insert Name: Bcr encoding residues 1004-1271
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:17116687
Vector Backbone Description: Vector Backbone:pGEX 3X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: We subcloned a Pvu II-EcoRI fragment into pGEX-3X digested with Sma I -EcoRI. This includes the natural stop codon and 3' untranslated sequences. The construct encodes the GTPase activating domain of Bcr which is active against GTP-bound Rac. The Pvu II site is located in the amino acid string -QLDP-. Constructed by Vesa Kaartinen. The Rosetta2 strain may improve yield of the fusion protein.
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Source: Addgene
