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URL: http://www.addgene.org/41072
Proper Citation: RRID:Addgene_41072
Insert Name: Tetherin
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:22130541
Vector Backbone Description: Backbone Marker:Len Neckers Laboratory (NCI, Bethesda, MD) (Koga et al. 2006, PMID: 16844778); Backbone Size:5400; Vector Backbone:pFLAG-Hsp90α; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid was generated by cloning of tetherin from plasmid pCMV-HA.Tetherin (Addgene plasmid #41068) into the BamHI and XhoI sites of pFLAG-Hsp90α to generate pFLAG-Tetherin (Addgene plasmid #41070). Serine substitutions at positions aa137 and aa144 were then generated by Quik Change II Site-directed Mutagenesis Kit (Strategene).
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Source: Addgene
