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URL: http://www.addgene.org/45477
Proper Citation: RRID:Addgene_45477
Insert Name: flaA-GFP
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10447882
Vector Backbone Description: Vector Backbone:pJB98; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: pflaG was generated by subcloning the 0.8kb XbaI/PstI fragment encoding GFP and T7 gene 10 rbs from pGFPmut3 into pJB98. The flaA promoter including its -35, -10, and transcriptional start sites were amplified from the chromosomal DNA of L. pneumophila 02 and ligated directly upstream of GFP using BamHI/XbaI. pJB98 was generated from pKB5 (Berger and Isberg, 1993) by deletion of ~1.5kb HpaI/EcoRI fragment containing the ptac promoter and lacIq gene.
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Source: Addgene
