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URL: http://www.addgene.org/45505
Proper Citation: RRID:Addgene_45505
Insert Name: PAI-RBP v2
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11001948
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pCEFL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Our lab isolated the protein based on it's ability to bind to a region of the PAI-1 (plasminogen activator inhibitor type 1) 3'UTR that we had determined to be involved in the cAMP regulation of mRNA stability. A search of the database revealed that the cDNA for the protein had been cloned by the German Genome Project and dubbed hypothetical protein. They kindly provided us with the clone, which we subcloned into pET-15b for expression of the protein and then into CMV-Flag for mammalian expression. Subsequently, we discovered isoforms of the protein with or without 6 and/or 15 amino acid insertions. Variant 2 has the 6 amino acid insertion but lacks the 15 amino acid insertion. Additional reference: Heaton, JH et al 2003 Thromb Haemost 89; 959-966.
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Source: Addgene
