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Plasmid Name
pBabe HA-Runx1-neo
RRID:Addgene_45815 RRID Copied  
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RRID:Addgene_45815
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Plasmid Information

URL: http://www.addgene.org/45815

Proper Citation: RRID:Addgene_45815

Insert Name: Runx1

Organism: Mus musculus

Bacterial Resistance: Ampicillin

Defining Citation: PMID:21873240

Vector Backbone Description: Backbone Marker:Weinberg Lab (Addgene plasmid 1767); Backbone Size:5300; Vector Backbone:pBabe neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin

Comments: pBabe HA-Runx1 neo was constructed by PCR using a mouse Runx1 template (Open Biosystems) and the following primers: GCGCAGATCTATGTACCCATACGACGTCCCAGACTACGCTGCTTCAGACAGCATTTTTGAGTC (forward), GCGCGAATTCTCAGAAGCATTCACAGTTTCC (reverse). The PCR product was gel purified, digested with BglII and EcoRI, and then ligated into pBabe neo, which had been digested with BamHI and EcoRI and then dephosphorylated.

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Data and Source Information

Source: Addgene