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URL: http://www.addgene.org/46086
Proper Citation: RRID:Addgene_46086
Insert Name: PpoI
Organism: physarum polycephalum
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10082660
Vector Backbone Description: Backbone Marker:clontech; Vector Backbone:pCMVbeta; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The I-PpoI expression vector pCNPpo6 was constructed from a cloned Physarum intron (pI3-941). The I-PpoI ORF was PCR-amplified, digested with HhaI and then ligated to an oligonucleotide adapter to add a new ATG start codon, an in-frame, N-terminal SV40 large T-antigen nuclear localization signal (nls)and flanking restriction cleavage sites. The resulting nls-Ppo fragment was ligated into the NotI site of pCMVbeta to generate pCNPpo6.
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Source: Addgene
