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URL: http://www.addgene.org/46140
Proper Citation: RRID:Addgene_46140
Insert Name: mCherry
Bacterial Resistance: Ampicillin
Defining Citation: PMID:22908272
Vector Backbone Description: Backbone Marker:stratagene; Vector Backbone:pBluescript II KS+; Vector Types:Cre/Lox, mutagenic gene trap for zebrafish; Bacterial Resistance:Ampicillin
Comments: mCherry is in the "+2" reading frame starting from the exon. This is a Tol2-based FlExTrap vector. It consists of a highly mutagenic gene trap contained within the FlEx cassette that can be stably inverted by both Cre and Flp recombinases. FRT/F3 and loxP/lox5171 are incompatible recombination sites used for the Flp and Cre recombinases, respectively, to stably invert the cassette. The reporter gene, mCherry, has its own initiation codon.
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Source: Addgene
