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Plasmid Name
pcDNA3.2 V5-DEST mir-1-1 reporter
RRID:Addgene_46646 RRID Copied  
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RRID:Addgene_46646
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Plasmid Information

URL: http://www.addgene.org/46646

Proper Citation: RRID:Addgene_46646

Insert Name: mir-1-1 reporter

Bacterial Resistance: Chloramphenicol and Ampicillin

Defining Citation: PMID:23415231

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression, RNAi, Expression of query pri-miRNA sequences in HEK293 cells; Bacterial Resistance:Chloramphenicol and Ampicillin

Comments: Query pri-miRNA sequences are to be recombined into this plasmid using Gateway recombination. The human pri-mir-1-1 sequence is cloned downstream of the query pri-miRNA and is transcriptionally fused to the query pri-miRNA sequence. This plasmid was used for ectopic pri-miRNA expression in HEK293 cells

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Ratings and Alerts

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Data and Source Information

Source: Addgene