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URL: http://www.addgene.org/47589
Proper Citation: RRID:Addgene_47589
Insert Name: PMCA4x/b
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:12624087
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: HPMCA4x/b coding sequence starts with amino acid 2 (Thr) at pos 1354. This plasmid carries two missense mutations that cause K442R and P663L in the amino acid sequence of hPMCA4b. The depositing lab did not notice any functional consequences in the assays they performed. However, these were mostly concerned with membrane localization and did not include any enzymatic functional studies. Thus, it is not know whether (and how) these two mutations affect the calcium-pumping enzymatic function of the encoded GFP-hPMCA4b protein.
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Source: Addgene
