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Plasmid Name
pET22b-EC12-129/138
RRID:Addgene_47926 RRID Copied  
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RRID:Addgene_47926
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Plasmid Information

URL: http://www.addgene.org/47926

Proper Citation: RRID:Addgene_47926

Insert Name: E-Cadherin EC12

Organism: Mus musculus

Bacterial Resistance: Ampicillin

Defining Citation: PMID:23923816

Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5406; Vector Backbone:pET-22b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin

Comments: Plasmid contains E-Cadherin domains 1 and 2 (aa157-375, when compared to GenBank reference sequence NP_033994.1). Numbering of the mutations is relative to this sequence. The pro-peptide sequence that is normally cleaved during maturation was intentionally removed as part of the cloning strategy for expression in bacteria.

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Data and Source Information

Source: Addgene