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URL: http://www.addgene.org/48282
Proper Citation: RRID:Addgene_48282
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Size:5767; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid is an empty destination vector. It is a polycistronic vector that can express up to five genes at once. Genes must first be cloned into any of our 8-series transfer vectors (8BT,8CT,8UT), then subcloned into any of the five cassettes of the destination vector: Cassette 1: BamHI/XbaI Cassette 2: AsiSI/PspXI Cassette 3: SbfI/AscI Cassette 4: AdeI/NotI Cassette 5: PacI/FseI Please note that you must always insert your gene into the lowest cassette number first (e.g., if you're using cassettes 2 and 3, you must put your gene into cassette 2 first, then move on to cassette 3). You do not have to use all of the cassettes. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/
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Source: Addgene
