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Plasmid Name
roGFP_iE_C147S_pQE30
RRID:Addgene_48631 RRID Copied  
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RRID:Addgene_48631
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Plasmid Information

URL: http://www.addgene.org/48631

Proper Citation: RRID:Addgene_48631

Insert Name: roGFP1

Organism: jellyfish

Bacterial Resistance: Ampicillin

Defining Citation: PMID:23589496

Vector Backbone Description: Backbone Size:3418; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin

Comments: This plasmids needs to be amplified and maintained in M15 at all times as this strain has the pREP4 repressor vector that prevents undesired expression (it's important to maintain the plasmid when the protein is not expressed even if it's believed to be inert for the bacteria, to avoid selective pressure in favour of recombinant versions of the gene).

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Data and Source Information

Source: Addgene