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URL: http://www.addgene.org/50351
Proper Citation: RRID:Addgene_50351
Insert Name: TRBP
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:23658827
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Fragment encoding a full length human TRBP was amplified using a cDNA mixture of total RNA extracted from human HeLa cells by PCR with primers containing restriction enzyme sites of HindIII and NotI. The amplified fragment was digested with the same enzyme sites and cloned into pcDNA3.1-myc/His A vector (Invitrogen). C-terminal myc-tagged TRBP fragment was amplified from pcDNA3.1-TRBP using primer with restriction enzyme sites NheI and HindIII. The amplified fragments were digested with same enzyme sites and cloned into pET-28a vector (Novagen).
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Source: Addgene
