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URL: http://www.addgene.org/51260
Proper Citation: RRID:Addgene_51260
Insert Name: 3xFLAG-dCas9
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25051498
Vector Backbone Description: Backbone Marker:Hodaka Fujii; Backbone Size:6238; Vector Backbone:pMXs-neo; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: The coding sequence of 3xFLAG-dCas9 can be cleaved with Pac I and Not I. Construction strategy of gRNA retroviral vectors 1. Cleave gBlock from a gRNA vector constructed using gRNA cloning vector (Addgene #41824) with appropriate restriction enzymes (eg. [Xho I + Hind III], EcoR I). 2. Insert the cleaved gBlock into pSIR-based self-inactivating retroviral vectors. Vectors & Sites of insertion pSIR-neo (Addgene #51128): eg. [Xho I + Hind III] pSIR-GFP (Addgene #51134): eg. [Xho I + Hind III], EcoR I pSIR-DsRed-Express2 (Addgene #51135): eg. [Xho I + Hind III], EcoR I pSIR-hCD2 (Addgene #51143): eg. EcoR I
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Source: Addgene
