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URL: http://www.addgene.org/51948
Proper Citation: RRID:Addgene_51948
Bacterial Resistance: Spectinomycin
Defining Citation: PMID:25188631
Vector Backbone Description: Vector Backbone:NA; Vector Types:; Bacterial Resistance:Spectinomycin
Comments: LB Lennox, at 32°C or below. Strain has a biotin auxotrophy, but this should not affect growth in rich media. It has 13 "essential" genes that end in TAG recoded to TAA, and its prfA gene has been deleted. Though viable, strain growth is slow. Genotype: ΔmutS:cat.Δ(ybhB-bioAB):[λcI857.Δ(cro-ea59):tetR-bla], ΔprfA, spec+
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Source: Addgene