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URL: http://www.addgene.org/55614
Proper Citation: RRID:Addgene_55614
Insert Name: 3xFLAG tagged HERC2 (C4762S)
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:24855649
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5137; Vector Backbone:pcDNA5 FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: There is a unique XhoI between the N-terminal 3xFLAG/STREP tag and the beginning of the HERC2 ORF. When cut with XhoI, PmeI, the untagged HERC2 ORF can be excised. There is a TEV-protease site inserted between the N-terminal 3xFLAG/STREP tag and the HERC2 ORF. Silent mutations have been incorporated into the ORF of HERC2 to render it refractory to the shRNA described in the associated publication.
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No rating or validation information has been found for pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R).
No alerts have been found for pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R).
Source: Addgene
