Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
URL: http://www.addgene.org/55792
Proper Citation: RRID:Addgene_55792
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12623879
Vector Backbone Description: Vector Backbone:pBluescript II SK+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The distance between the two Not1 sites is ~6kb Clone into the Sal1 site (or Sal/Hind3 site). Release the pBluescript backbone with Not1 for transgenics. This construct consists of a full-length α-MHC promoter in which 3 GATA sites and 2 TREs were ablated. Other cis-acting regions important for cardiac-specific expression were left intact. To make the attenuated promoter Tet responsive, 7 repeats of the TetO sequences were inserted adjacent to the TATA box. The human growth hormone polyadenylation signal was placed downstream from the unique cloning site. Any gene of interest inserted into this Tet-responsive promoter construct will be inducible.
Expand AllWe found {{ ctrl2.mentions.all_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
A list of researchers who have used the resource and an author search tool
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for pJG/ Inducible ALPHA MHC.
No alerts have been found for pJG/ Inducible ALPHA MHC.
Source: Addgene