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Plasmid Name
pJG/ Inducible ALPHA MHC
RRID:Addgene_55792 RRID Copied  
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RRID:Addgene_55792
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Plasmid Information

URL: http://www.addgene.org/55792

Proper Citation: RRID:Addgene_55792

Bacterial Resistance: Ampicillin

Defining Citation: PMID:12623879

Vector Backbone Description: Vector Backbone:pBluescript II SK+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Comments: The distance between the two Not1 sites is ~6kb Clone into the Sal1 site (or Sal/Hind3 site). Release the pBluescript backbone with Not1 for transgenics. This construct consists of a full-length α-MHC promoter in which 3 GATA sites and 2 TREs were ablated. Other cis-acting regions important for cardiac-specific expression were left intact. To make the attenuated promoter Tet responsive, 7 repeats of the TetO sequences were inserted adjacent to the TATA box. The human growth hormone polyadenylation signal was placed downstream from the unique cloning site. Any gene of interest inserted into this Tet-responsive promoter construct will be inducible.

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Data and Source Information

Source: Addgene