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URL: http://www.addgene.org/59205
Proper Citation: RRID:Addgene_59205
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:17038565
Vector Backbone Description: Backbone Marker:PMID 9733856 ; Vector Backbone:HIV-CS-CG; Vector Types:Mammalian Expression, Lentiviral, DamID; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The V5 epitope tag serves as a linker between Dam and your favorite protein. The lentiviral vectors can be used in two ways. When transfected as a conventional plasmid, the strong viral promoter drives high expression of the Dam(fusion) proteins. This should not be used for DamID experiments, but it is useful for checking of the proteins by Western blotting or immunofluorescence microscopy. When used as lentivirus, the strong promoter is deleted upon integration of the virus, and expression of the Dam(fusion) is now driven by the weak pIND-derived heatshock promoter.
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Source: Addgene
