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URL: http://www.addgene.org/61089
Proper Citation: RRID:Addgene_61089
Insert Name: H1 promoter; gRNA sequences
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25209046
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, /Cas9 gRNA cloning vector; Bacterial Resistance:Ampicillin
Comments: As Ampicillin gene and H1 promoter contain BsaI sites, we mutated the sequence of Amp gene (G1601C, not change amino acid), and mutated H1 promoter from GAGACC to GAGGACC to eliminate the BsaI internal site.
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Source: Addgene
