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URL: http://www.addgene.org/62862
Proper Citation: RRID:Addgene_62862
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:25897682
Vector Backbone Description: Vector Backbone:pCC1BAC; Vector Types:Synthetic Biology, Episomal vector for Phaeodactylum tricornutum; Bacterial Resistance:Chloramphenicol
Comments: Contains yeast URA3 and HIS3 genes for selection in yeast. Contains ShBle cassette for selection in Phaeodactylum tricornutum. Depositor states that the I-SceI restriction site in this plasmid (5’-AGTTACGCTAGGGATAACAGGTAATATAG) varies from the NEB listed recognition sequence and may reduce the efficiency of cleavage using this enzyme.
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Source: Addgene