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Plasmid Name
pAraH6HATT
RRID:Addgene_63901 RRID Copied  
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RRID:Addgene_63901
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Plasmid Information

URL: http://www.addgene.org/63901

Proper Citation: RRID:Addgene_63901

Insert Name: TT Fab

Organism: Homo sapiens

Bacterial Resistance: Chloramphenicol

Vector Backbone Description: Backbone Size:3500; Vector Backbone:pAra; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol

Comments: Provided with the pComb3H system for arabinose induced expression of soluble protein with detection tags. The “TT” refers to the human Fab to tetanus toxin which can be used as a protein expression control. Alternatively, one could upgrade to the pComb3X system, also available on Addgene, which already contains tags. Select reference: Cary, S.P.; Lee, J.; Wagenknecht, R.; Silverman, G.J. (2000) Characterization of Superantigen-Induced Clonal Deletion with a Novel Clan III-Restricted Avian Monoclonal Antibody: Exploiting Evolutionary Distance to Create Antibodies Specific for a Conserved VH Region Surface. J. Immun., 164, 4730-4741.

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Data and Source Information

Source: Addgene