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URL: http://www.addgene.org/66201
Proper Citation: RRID:Addgene_66201
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25917172
Vector Backbone Description: Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin
Comments: Accession number KR029100 By Omega-PCR cloning, an E. coli promoter-LacZ sequence (198 bp in length) was inserted. The LacZ marker gene can be used to facilitate selection of Escherichia coli positive CRISPR/Cas9 clones carrying the sgRNA expression cassettes. Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
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Source: Addgene