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URL: http://www.addgene.org/67717
Proper Citation: RRID:Addgene_67717
Insert Name: Hexahistidine/Maltose binding protein/MS2 coat protein fusion
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:17548432
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5422; Vector Backbone:pET30b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: The MS2 coat protein in this plasmid contains the V29/dIFG mutations described in Lim and Peabody 1994 (Nucleic Acids Res. 1994 Sep 11;22(18):3748-52) which are reported to prevent coat protein multimerization and increase affinity for RNA.
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Source: Addgene