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URL: http://www.addgene.org/68051
Proper Citation: RRID:Addgene_68051
Insert Name: KaiC
Organism: Synechococcus elongatus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:23071342
Vector Backbone Description: Vector Backbone:pET41a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PreScission (GE Healthcare) Protease cutting site is introduced before KaiC. KaiC is cloned between NcoI and HiNdII. Use BL21DE3 and GST column for protein purification. Use PreScission Protease to cut GST tag off.
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Source: Addgene
