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URL: http://www.addgene.org/71538
Proper Citation: RRID:Addgene_71538
Insert Name: Cas9
Organism: S. pyogenes
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:26141150
Vector Backbone Description: Vector Backbone:pSC101ts; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Comments: The plasmid curing system consists of an arabinose inducible promoter, PBAD to express a gRNA targeting the bla gene on the gRNA plasmid. When induced, the bla-targeting gRNA is expressed, leading Cas9 to cleave the gRNA plasmid and resulting in plasmid elimination.
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Source: Addgene
