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URL: http://www.addgene.org/73144
Proper Citation: RRID:Addgene_73144
Insert Name: sHRPb-NLG
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:27240195
Vector Backbone Description: Backbone Size:4247; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: sHRPb is the small split HRP fragment. It consists of amino acids 214-308 of horseradish peroxidase (HRP) with the following 2 mutations: N255D, L299R The insert contains the following features: EcoRI-NLG1 ss-AgeI-sHRPb-5 aa linker-V5 epitope tag-MfeI-NLG1-stop-BglII-NotI CAG promoter 5 aa linker: GSGSG V5 epitope tag: GKPIPNPLLGLDST NLG: mouse neuroligin1 This construct was derived from the 3xAP-NLG1 plasmid (Addgene ID 43923).
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Source: Addgene
