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URL: http://www.addgene.org/73151
Proper Citation: RRID:Addgene_73151
Insert Name: sHRPa-Aga1P
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:27240195
Vector Backbone Description: Backbone Size:4781; Vector Backbone:YIP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: sHRPa is the large split HRP fragment. It consists of amino acids 1-213 of horseradish peroxidase (HRP) with the following 4 mutations: T21I, P78S, R93G, N175S. The insert contains the following features: BamHI-Aga1P ss-AflII-sHRPa-3 aa linker-V5 epitope tag-9 aa linker-NheI-Aga1P mature-stop-XhoI glyceraldehyde-3-phosphate dehydrogenase GPD promoter (constitutive) 3 aa linker: GSG 9 aa linker: GSGGGGSSG V5 epitope tag: GKPIPNPLLGLDST This plasmid was derived from the previously published “LAP-Aga1P” plasmid (White and Zegelbone, Biochemistry, 52, 3728-3739, 2013). When this vector is linearized by restriction digest, it incorporates into the yeast genome.
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Source: Addgene
