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URL: http://www.addgene.org/73152
Proper Citation: RRID:Addgene_73152
Insert Name: Aga2P-HRP
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:27240195
Vector Backbone Description: Backbone Size:55791; Vector Backbone:pCTCON2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: HRP = full-length horseradish peroxidase. This vector contains the following features: EcoRI-Aga2P-HA-TEVs-15 aa linker-NheI-HRP-3 aa linker-BamHI-myc-stop-XhoI GAL1 promoter (galactose inducible) 15 aa linker: GGGGSGGGGSGGGGS 3 aa linker: GSG HA: YPYDVPDYA myc: EQKLISEEDL TEVs: ENLYFQG, cleavage recognition site for TEV protease This construct is derived from the previously reported Aga2P-HRP construct (Lam et. al. Nature Methods, 12, 51-54, 2015), except the codon optimization of the synthetic HRP gene is different in this construct.
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Source: Addgene
