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URL: http://www.addgene.org/74014
Proper Citation: RRID:Addgene_74014
Insert Name: Psd95 shRNA
Organism: Rattus norvegicus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16815335
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: Map: FHUG+W-HinDIII-SalI-XbaI-(p95b)-KpnI-SacII-PspOMI-SmaI-BamHI-(GFP)-BglII-BsrBI-EcoRV-HinDIII-ClaI Also associated with Liu, et al. J Neurophysiol. 2014 Feb;111(3):648-58. doi: 10.1152/jn.00262.2013 PMID 24225540. Psd95 shRNA expressed under control of H1 promoter. Psd95 coding sequence modified to GGATATTCAAGCGCATAAG make it resistant to the shRNA. Psd95-EGFP fusion protein expressed under control of UbC promoter. The publication by Schluter et al linked above describes the creation of the beta isoform of Psd95 in detail
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Source: Addgene
