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URL: http://www.addgene.org/83027
Proper Citation: RRID:Addgene_83027
Insert Name: shNKCC1
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:23015452
Vector Backbone Description: Backbone Marker:Jacks Lab (MIT); Backbone Size:7566; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: This plasmid contains a c4351t substitution within the shRNA. The depositing lab states that they do not know whether this mutation affects plasmid function. pSico allows for conditional (Cre-Lox), stable expression of shNKCC1. Cre expression excises GFP and allows for shNKCC1 expression.
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Source: Addgene
