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URL: http://www.addgene.org/83479
Proper Citation: RRID:Addgene_83479
Insert Name: DLD
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:29128334
Vector Backbone Description: Backbone Marker:PMID: 26186194; Backbone Size:10056; Vector Backbone:pPHAGE C-TAP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Mutation (first described in PMID: 17404228) was created using the method described in PMID: 17446874. Two outer (F: CTCTTGGTTTGCATTGGCCG, IRES_R: CCTCACATTGCCAAAAGACG) and two mutation-site primers were used. The mutated PCR product and the backbone vector were digested using EcoRI, gel purified and ligated. Note that the mutation is numbered in accordance with the original publication (PMID: 17404228), which lacked the mitochondrial targeting signal (residues 1-35). In this full-length protein, it is found at position 491.
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Source: Addgene