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Plasmid Name
pPHAGE_DLD-S456A-C-TAP
RRID:Addgene_83479 RRID Copied  
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RRID:Addgene_83479
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Plasmid Information

URL: http://www.addgene.org/83479

Proper Citation: RRID:Addgene_83479

Insert Name: DLD

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:29128334

Vector Backbone Description: Backbone Marker:PMID: 26186194; Backbone Size:10056; Vector Backbone:pPHAGE C-TAP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin

Comments: Mutation (first described in PMID: 17404228) was created using the method described in PMID: 17446874. Two outer (F: CTCTTGGTTTGCATTGGCCG, IRES_R: CCTCACATTGCCAAAAGACG) and two mutation-site primers were used. The mutated PCR product and the backbone vector were digested using EcoRI, gel purified and ligated. Note that the mutation is numbered in accordance with the original publication (PMID: 17404228), which lacked the mitochondrial targeting signal (residues 1-35). In this full-length protein, it is found at position 491.

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Data and Source Information

Source: Addgene