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URL: http://www.addgene.org/86385
Proper Citation: RRID:Addgene_86385
Insert Name: D. melanogaster ncm enhancer
Organism: Drosophila melanogaster
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:28024147
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)
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Source: Addgene
