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Plasmid Name
pRB2958
RRID:Addgene_8676 RRID Copied  
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RRID:Addgene_8676
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Plasmid Information

URL: http://www.addgene.org/8676

Proper Citation: RRID:Addgene_8676

Insert Name: tub1-827 under GAL1 promoter

Organism: Saccharomyces cerevisiae

Bacterial Resistance: Ampicillin

Defining Citation: PMID:11739794

Vector Backbone Description: Backbone Size:0; Vector Backbone:pTS210; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin

Comments: tub1-827 was from Richards et al 2000 Mol. Biol. Cell 11, 1887-1903. It was PCRed, and the PCR products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210. The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.

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Data and Source Information

Source: Addgene