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URL: http://www.addgene.org/87746
Proper Citation: RRID:Addgene_87746
Insert Name: T4 DNA ligase
Organism: Other
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:23754529
Vector Backbone Description: Backbone Marker:Kitagawa et al. (PMID:16769691); Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: For complete cloning information, please see the paper's supplement. For expression, E. coli DH5a-E cells harbouring each expression vector were grown at 37°C in LB broth containing chloramphenicol (34 µg.ml-1), to OD600 ~ 0.6. IPTG (0.4 mM, final concentration) was added as an inducer, and protein over-expression was at 28°C for 16-18 h.
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Source: Addgene
