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Plasmid Name
pRK603
RRID:Addgene_8831 RRID Copied  
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RRID:Addgene_8831
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Plasmid Information

URL: http://www.addgene.org/8831

Proper Citation: RRID:Addgene_8831

Insert Name: TEV protease, S219D mutant

Organism: tobacco etch virus

Bacterial Resistance: Kanamycin

Defining Citation: PMID:10873547

Vector Backbone Description: Backbone Marker:Herman Bujard Lab; Backbone Size:6700; Vector Backbone:pZA31; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin

Comments: This TEV protease expression vector is intended to be used for controlled intracellular processing of fusion proteins (TEV protease substrates) in E. coli. Expression of the TEV protease gene on pRK603 will be constitutive unless the host strain produces Tet repressor. Regulated expression can be achieved in DH5alphaPro or BL21Pro cells (Clontech).

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Data and Source Information

Source: Addgene