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URL: http://www.addgene.org/8831
Proper Citation: RRID:Addgene_8831
Insert Name: TEV protease, S219D mutant
Organism: tobacco etch virus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:10873547
Vector Backbone Description: Backbone Marker:Herman Bujard Lab; Backbone Size:6700; Vector Backbone:pZA31; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This TEV protease expression vector is intended to be used for controlled intracellular processing of fusion proteins (TEV protease substrates) in E. coli. Expression of the TEV protease gene on pRK603 will be constitutive unless the host strain produces Tet repressor. Regulated expression can be achieved in DH5alphaPro or BL21Pro cells (Clontech).
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Source: Addgene