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Plasmid Name
pET21-Flavidin
RRID:Addgene_89881 RRID Copied  
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RRID:Addgene_89881
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Plasmid Information

URL: http://www.addgene.org/89881

Proper Citation: RRID:Addgene_89881

Insert Name: Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag

Organism: E. coli

Bacterial Resistance: Ampicillin

Defining Citation: PMID:28757182

Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin

Comments: The residue numbering is based 1986 paper from Charles Cantor: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC339579 (see Figure 3) In the original sequence, there is a signal peptide and N-terminal sequence that are proteolytically processed. Thus, the most common version is Core Streptavidin, which starts at residue 13 and ends at residue 139, as shown here: http://www.jbc.org/content/270/47/28204.long (see Figure 1)

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Data and Source Information

Source: Addgene