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URL: http://www.scienceexchange.com/facilities/protein-core-facility-columbia
Proper Citation: Columbia University Protein Core Facility (RRID:SCR_012597)
Description: THIS RESOURCE IS NO LONGER IN SERVICE. Documented on April 25,2024. Columbia University Protein Core Facility provides a range of services to all Columbia researchers and to outside users, including mass spectrometry, protein sequencing, and DNA sequencing. Mass Spectrometry: Services available include protein identification from gel bands or spots by MALDI-TOF and ESI-LC-MS/MS, post-translational modification identification, and mass analysis of intact proteins. Mass spectrometry is used to identify interacting partners in protein complexes that have been purified by immunoprecipitation and other methods. The Core also routinely provides structural confirmation of expressed proteins and protein fragments for structural biology studies. Protein Sequencing: N-terminal sequencing of proteins in solution or blotted to PVDF membranes is available. DNA Sequencing: The core offers high quality, fast turnaround DNA sequencing of various types DNA - plasmids, PCR products, GC-rich templates, cosmids, and BACs. DNA sequencing results are distributed through a secure network server with automated email notification.
Abbreviations: Columbia Protein Core Facility
Resource Type: service resource, access service resource, core facility
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