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URL: https://code.google.com/p/sasqpcr/
Proper Citation: SASqPCR (RRID:SCR_003056)
Description: All-in-one computer program for robust and rapid analysis of quantitative reverse transcription real-time polymerase chain reaction (RT-qPCR) data in SAS. It incorporates all functions important for RT-qPCR data analysis including assessment of PCR efficiencies, validation of internal reference genes and normalizers, normalization of confounding variations across samples and statistical comparisons of target gene expression in parallel samples. The program is highly automatic in data analyses and result output. The input data have no limitations for the number of genes or cDNA samples. Users can simply change the macro variables to test various analytical strategies, optimize results and customize the analytical processes. The program is also extendable allowing advanced SAS users to develop particular statistical tests appropriate for their experimental designs. Thus users are the actual decision-makers controlling RT-qPCR data analyses. The program has to be used in SAS software; however, extensive SAS programming knowledge is not required.
Synonyms: SASqPCR: robust and rapid analysis of RT-qPCR data in SAS
Resource Type: software resource
Defining Citation: PMID:22238653
Keywords: standalone software, computation, analysis, statistics, rt-qpcr, cdna, mrna, gene expression, quantification, reference gene, normalization, sas
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