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URL: https://code.google.com/p/mpprimer/
Proper Citation: MPprimer (RRID:SCR_003063)
Description: A software program for reliable multiplex PCR primer design. It employs the widely used primer design program Primer3 and the primer specificity evaluation program MFEprimer to design and evaluate the candidate primers based on genomic or transcript DNA database, followed by careful examination to avoid primer dimerization. The graph-expanding algorithm derived from the greedy algorithm was used to determine the optimal primer set combinations (PSCs) for multiplex PCR. In addition, it provides a virtual electrophotogram to help users choose the best PSC. It is a valuable tool for designing specific, no dimer formation and amplicons size constrained PSCs to improve the multiplex PCR experiments.
Synonyms: MPprimer: a program for reliable multiplex PCR primer design
Resource Type: software resource, analysis service resource, service resource, production service resource, data analysis service
Defining Citation: PMID:20298595
Keywords: standalone software, pcr, multiplexpcr, mfeprimer, primer3, specificity, dimer, primer design
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