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URL: http://seq.cbrc.jp/recount/
Proper Citation: RECOUNT (RRID:SCR_006118)
Description: THIS RESOURCE IS NO LONGER IN SERVICE, documented on 5/29/14. An Expectation Maximization error correction tool for next generation sequencing data (Solexa/Illumina). The main features of RECOUNT: * Uses quality score to estimate the correct counts, hence potentially more accurate. * It does not use reference genome. * Memory efficient. Next generation sequencing technologies enable rapid, large-scale production of sequence data sets. Unfortunately these technologies also have a non-neglible sequencing error rate, which biases their outputs by introducing false reads and reducing the quantity of the real reads. They have applied RECOUNT to several types of Solexa/Illumina reads from mouse embryo, 5''-end SAGE, and bacterial metagenomic reads. They found that the correction by the tool not only increases the number of mappable reads, but also makes a real difference in the biological interpretation of next generation sequencing data.
Abbreviations: RECOUNT
Resource Type: software resource, software application, data processing software
Defining Citation: PMID:20180274
Keywords: next generation sequencing, c++, error correction, sequence count
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National Institute of Advanced Industrial Science and Technology |
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