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URL: http://www.scienceexchange.com/facilities/w-m-keck-biomedical-mass-spectrometry-laboratory-virginia
Proper Citation: UVa W.M. Keck Biomedical Mass Spectrometry Laboratory (RRID:SCR_012589)
Description: THIS RESOURCE IS NO LONGER IN SERVICE. Documented on April 15,2024. W.M. Keck Biomedical Mass Spectrometry Laboratory within the Biomolecular Research Facility identifies and analyzes proteins from gels and solution using current mass spectrometry techniques. There are techniques to compare samples which have different amounts of the same proteins. Analyses available: - Protein identification and sequencing from gel or solution by either peptide mass fingerprinting or ESI-LC/MS/MS at the low femtomole level. - Analysis of protein mixtures such as tissue and media analysis of tissue samples prepared by Laser Capture Microdissection - Proteomics, comparison of proteins in tissue samples using chemical/isotopic labels or label free analysis - Absolute quantitation of proteins using labeled peptides and selective reaction monitoring - Identification of phosphorylation sites using titanium dioxide enrichment - Identification of post-translational modifications (deamination, ubiquitination, etc) - De Novo (manual) sequence analysis of novel proteins to obtain sufficient sequence data for cloning - High resolution, high mass accuracy measurements of peptides and small proteins - Confirmation of peptide identity by MALDI mass measurements - Identification of binding partners Equipment Our current mass spectrometers: - Thermo Scientific LTQ Orbitrap Velos for high accuracy, high sensitivity analysis - Bruker Microflex for mass analysis by MALDI - Thermo Scientific TSQ Quantum Access MAX triple quadrupole instrument Sample types analyzed: - gel bands, stained with Coomassie, silver, fluorescent stains - proteins in solution - tissue samples - media - Laser Capture Microdissection tissue samples.
Abbreviations: UVa W.M. Keck Biomedical Mass Spectrometry Laboratory
Synonyms: University of Virginia School of Medicine W.M. Keck Biomedical Mass Spectrometry Laboratory
Resource Type: service resource, core facility, access service resource
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