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University of Florida ICBR Gene Expression and Genotyping Core Facility (RRID:SCR_019145)Copy Citation Copied
URL: http://www.biotech.ufl.edu/cores/gene-expression-genotyping/
Proper Citation: University of Florida ICBR Gene Expression and Genotyping Core Facility (RRID:SCR_019145)
Description: Core provides services and consultation on Single Cell RNA-Seq, RNA-Seq, 16s metagenomics and Affymetrix gene expression arrays. High-throughput RNA-seq libraries, 16s libraries and sequencing capture libraries can be done by using Agilent Bravo robot. QuantiGene RNA Assays measure up to 80 gene targets directly with degraded and cross-linked RNA in FFPE tissues and blood, with no RNA purification required.BioRad QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules with greater precision and sensitivity than qPCR, sensitivity off ddPCR System can facilitate expanded analysis of single cells. Genotyping services include fragment analysis using AB3730, 96 capillary technology, development of microsatellite libraries using Illumina sequence data, and genotyping using mouse tails or ear punches.
Synonyms: University of Florida ICBR Gene Expression and Genotyping, UF ICBR Gene Expression & Genotyping
Resource Type: service resource, access service resource, core facility
Keywords: USEDit, gene expression, genotyping, single cell RNAseq, PCR, qPCR, analysis, ABRF
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