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University of Nebraska Lincoln Nebraska Center for Biotechnology Proteomics and Metabolomics Core Facility (RRID:SCR_021314)Copy Citation Copied
URL: https://biotech.unl.edu/proteomics-and-metabolomics#tab1
Proper Citation: University of Nebraska Lincoln Nebraska Center for Biotechnology Proteomics and Metabolomics Core Facility (RRID:SCR_021314)
Description: Provides services using mass spectrometry, including identification and relative quantification of proteins and several advanced methods for profiling and quantitation of small molecules.Provides outreach and education program including workshops,seminars and courses.Proteomics services include Bottom-up Proteomics of whole organisms, organs, organelles, tissues, cell pellets, pulldowns, complex or single protein mixes from solutions or gels;Quantification by label-free or multiplexed labeling techniques with microscale subfractionation to increase coverage;Post Translational Modification Characterization especially phosphopeptide enrichment from whole organism digests, but also ubiquitylation, acetylation, etc.Metabolomics services include Untargeted Quantification of small molecules;Primary metabolism using GC-MS and NIST/Fiehn Libraries;Secondary metabolism using LC-MS and Progenesis QI, NIST Library, mzCloud;Targeted Quantification using HPLC or LCMS.
Abbreviations: PMF
Synonyms: UNL Proteomics and Metabolomics Facility
Resource Type: service resource, access service resource, core facility
Keywords: USEDit, ABRF, mass spectrometry, protein identification, protein relative quantification, small molecules, proteomics, metabolomics
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